Platelet-Rich Plasma Improves Sperm DNA Integrity and Sperm function through Field Fertility trials of Cryopreserved Goat Semen

The preservation of sperm genomic integrity is critical for successful fertilization and subsequent embryonic development following artificial insemination. Cryopreservation-associated oxidative stress can compromise sperm DNA and may ultimately reduce reproductive efficiency. The present study evaluated the effect of platelet-rich plasma (PRP) supplementation on sperm DNA integrity and assessed its potential reproductive benefit under field conditions. Goat semen ejaculates were extended with TRIS egg yolk citrate extender supplemented with 0% PRP (control), 5% PRP (T1), 10% PRP (T2), and 15% PRP (T3), followed by cryopreservation and storage in liquid nitrogen (−196°C) for 72 h. Post-thaw samples were evaluated for sperm DNA fragmentation, and the treatment demonstrating the most favorable overall sperm response was subsequently evaluated through field artificial insemination trials. Spermatozoa supplemented with 10% PRP exhibited significantly lower DNA fragmentation compared with the control and other treatment groups, indicating enhanced preservation of genomic integrity during cryopreservation. In the field trial, artificial insemination using PRP-supplemented semen resulted in a conception rate of 41.52%, compared with 38.03% in the control group. The concurrent improvement in sperm DNA integrity and conception outcome suggests that PRP-mediated cellular protection may contribute to improved reproductive competence of cryopreserved spermatozoa. These findings provide a link between preservation of sperm genomic integrity and field reproductive performance and highlight 10% PRP as a promising biological additive for enhancing the quality and fertility potential of cryopreserved goat semen.